Searching for a genetic engineer specializing in gene knock-in techniques to perform genetic editing on hematopoietic stem cells. We received funding for in-vivo research which may lead to clinical breakthrough and FDA approval.
Key Responsibilities
- Design and execute gene knock-in strategies for CD34+ hematopoietic stem cells
- Optimize protocols for efficient gene editing in CD34+ cells using CRISPR/Cas9 or other advanced gene-editing technologies
- Conduct pre-clinical studies to assess the efficacy and safety of engineered CD34+ cells
- Analyze and interpret complex genomic data from gene-edited cells
- Collaborate with multidisciplinary teams including molecular biologists, bioinformaticians, and clinical researchers
- Maintain detailed laboratory records and prepare technical reports
- Stay current with the latest advancements in gene editing technologies and stem cell research
Qualifications
- Ph.D. in Molecular Biology, Genetics, Bioengineering, or a related field
- Minimum of 3 years of hands-on experience with gene editing techniques, preferably in hematopoietic stem cells
- Proven track record of successful gene knock-ins in primary human cells
- Expertise in CD34+ cell isolation, culture, and manipulation
- Proficiency in CRISPR/Cas9 or other gene-editing systems
- Experience with flow cytometry and cell sorting techniques
- Strong analytical skills and familiarity with genomic analysis tools
- Excellent organizational and communication skills
Preferred Qualifications
- Post-doctoral research experience in stem cell biology or gene therapy
- Experience with lentiviral vector design and production
- Familiarity with good manufacturing practices (GMP) for cell therapies
- Knowledge of regulatory requirements for pre-clinical and clinical trials